860336

3,3′,5,5′-Tetramethylbenzidine

≥99%

• Synonym(s): BM blue, Sure Blue TMB, TMB, TMB Blotting Plus, TMB substrate
• Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
• CAS Number: 54827-17-7
• Molecular Weight: 240.34
• Beilstein: 2808541
• EC Number: 259-364-6
• MDL number: MFCD00007748
• PubChem Substance ID: 24888505

Properties

• Quality Level: 200
• assay: ≥99%
• form: powder
• mp: 168-171 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

General description

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.

Packaging

100 mg in glass bottle

1, 5, 25 g in glass bottle

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H302 - H351 - H413
• Precautionary Statements: P202 - P264 - P270 - P273 - P301 + P312 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves