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TOYOPEARL® HW-40F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm

同義詞:

TOYOPEARL® HW-40F

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About This Item

分類程式碼代碼:
41115700

描述

HW-40F

產品線

TOYOPEARL®

形狀

slurry

包裝

bottle of 500 mL

參數

3 bar max. pressure

技術

HPLC: suitable

基質

hydroxylated methacrylic polymer

基質活性組

hydroxylated methacrylate phase

粒徑

30-60 μm

孔徑

100-7000 Da MW range

工作pH值

2-13

分離技術

size exclusion (SEC)

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一般說明

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

應用

Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

規格

Clean in place with 0.5 M NaOH or 0.1 M HCl.

外觀

Shipped in 20% (v/v) ethanol.

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法律資訊

Toyopearl is a registered trademark of Tosoh Corporation

象形圖

Flame

訊號詞

Warning

危險聲明

危險分類

Flam. Liq. 3

水污染物質分類(WGK)

WGK 1

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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分析證明 (COA)

Lot/Batch Number

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Francesca Magnani et al.
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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