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Merck
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Key Documents

G2580

Sigma-Aldrich

Sephadex® G-25

Fine

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

品質等級

技術

buffer exchange: suitable

基質活性組

phase

膨潤

1 g swells to 4-6 mL

珠尺寸

20-80 μm

應用

life science and biopharma

相容性

Cytiva

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1

InChI 密鑰

BCPVZFFJGCURNR-OCOFDJSDSA-N

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一般說明

Sephadex®是珠状凝胶过滤介质,由葡聚糖与环氧氯丙烷在碱性条件下交联制备而成。 用于亲和层析、蛋白层析和凝胶过滤层析。
分级分离范围(MW)
球状蛋白:1,000 - 5,000
右旋糖苷:100 - 5,000

應用

Sephadex® G-25 用于尺寸排阻层析,去除标记蛋白中的多余染料。

其他說明

G10050-100G′更新的产品编号为GE17-0032-01
G10050-500G′更新的产品编号为GE17-0032-02

法律資訊

Sephadex is a registered trademark of Cytiva

取代透過

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Celine Hoffmann et al.
Analytical biochemistry, 372(1), 62-71 (2007-09-11)
Fluorescein isothiocyanate (FITC) is a well-known probe for labeling biologically relevant proteins. However, the impact of the labeling procedure on protein structure and biological activities remains unclear. In this work, FITC-labeled human plasma fibronectin (Fn) was developed to gain insight
Jennifer Belyea et al.
Biochemistry, 45(48), 14275-14284 (2006-11-30)
The study of axial ligation by anionic ligands to ferric heme iron by resonance Raman spectroscopy provides a basis for comparison of the intrinsic electron donor ability of the proximal histidine in horse heart myoglobin (HHMb), dehaloperoxidase (DHP), and horseradish
Conformations of a metastable SH3 domain characterized by smFRET and an excluded-volume polymer model.
Mazouchi A, et al.
Biophysical Journal, 110(7), 1510-1522 (2016)
P M G Paiva et al.
Bioresource technology, 88(1), 75-79 (2003-02-08)
A highly purified trypsin inhibitor was obtained from Echinodorus paniculatus when an extract prepared from E. paniculatus seed flour (25 gl(-1), with 0.1 M ammonium acetate buffer, pH 8.3, under agitation for 6 min at 28 degrees C) was chromatographed
Zhifeng Huang et al.
Journal of biotechnology, 142(3-4), 242-249 (2009-05-30)
Recombinant human keratinocyte growth factor 2 (rhKGF-2) is a member of fibroblast growth factor protein family currently being investigated for its promising significant effects in treating epithelial damage. Molecular modification with polyethylene glycol (PEGylation) is an effective approach to improve

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