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Merck
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Key Documents

G5080

Sigma-Aldrich

葡聚糖凝胶 ® G-50

Fine

同義詞:

Sephadex G-50细粒葡聚糖树脂, 凝胶过滤树脂

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About This Item

CAS號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

品質等級

技術

affinity chromatography: suitable

基質活性組

phase

膨潤

1 g swells to 9-11 mL

珠尺寸

20-80 μm

應用

life science and biopharma

相容性

Cytiva

一般說明

Sephadex G-50(细颗粒)葡聚糖凝胶是广泛使用的凝胶过滤树脂,设计用于分子量30,000以上生物分子的脱盐和缓冲液置换。因其胶珠较小而具有高效率。不同类型的Sephadex的交联度不同,因此其溶胀程度和分子分离范围也不同。Sephadex G-50属于G型,G型Sephadex的型号包括从小分子G-10到大分子G-75的五种型号。Sephadex G-50分为4种不同粒径,包括粗(Coarse)、中(Medium)、细(Fine)和超细(Superfine)颗粒。
Sephadex® G-50是一种用于亲和层析、蛋白质层析和凝胶过滤层析的凝胶过滤介质。Sephadex 是一种由葡聚糖和环氧氯丙烷交联而成的珠状凝胶。
分馏范围(MW)
球状蛋白质:1,500-30,000
葡聚糖:500-10,000

應用

Sephadex® G-50 适用于:

  • 低分子量和高分子量分子的分离
  • 亲和色谱法、蛋白质色谱法和凝胶过滤色谱法
Sephadex® G-50葡聚糖凝胶适于:
  • 去除脂质体悬液中的非包埋羧基荧光素(CF)
  • 通过离心式尺寸排阻色谱纯化人MN-14单克隆抗体(mAb)
  • 对5和6部分洗脱的重组蛋白进行脱盐

特點和優勢

  • 一步完成脱盐、除污染和置换新缓冲液。
  • 适于通过凝胶过滤纯化DNA,除去小分子。
  • 凝胶珠较小,扩散距离更短。
  • 经典的凝胶过滤树脂。

  • Sephadex 脱盐被认为优于透析,因为它节省时间,稀释系数低,即便使用少量样品也能恢复高活性

其他說明

G5080-100G′更新后的产品编号为 GE17-0042-01
G5080-500G′更新后的产品编号为 GE17-0042-02

法律資訊

Sephadex is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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L M Weiner et al.
Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
B P Brunk et al.
Genetics, 124(1), 145-156 (1990-01-01)
The isolation of gain of function mutations has allowed the identification of a number of genes which are important in the normal development of the organism. We report here the isolation and characterization of Aristapedioid, a gain of function mutation
Jennifer E Dawson et al.
Nature communications, 11(1), 3146-3146 (2020-06-21)
Phosphorylation of intrinsically disordered eIF4E binding proteins (4E-BPs) regulates cap-dependent translation by weakening their ability to compete with eIF4G for eIF4E binding within the translation initiation complex. We previously showed that phosphorylation of T37 and T46 in 4E-BP2 induces folding

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