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重要文件

A50120

Sigma-Aldrich

DEAE-葡聚糖凝胶®

同義詞:

交葡聚糖凝胶®

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About This Item

CAS號碼:
MDL號碼:
分類程式碼代碼:
47101511
NACRES:
NA.56

形狀

powder

品質等級

技術

protein purification: suitable

基質

cross-linked dextran

珠尺寸

40-125 μm (dry)

孔徑

~200,000 Da exclusion limit

pH值

2—12

容量

3-4 meq/g binding capacity

一般說明

A50120-100G的更新产品编号为 GE17-0180-01
A50120-500G的更新产品编号为 GE17-0180-02

應用

DEAE葡聚糖凝胶可用于纯化和分离蛋白质的离子交换色谱。它已被用于鉴别和标记急性心肌梗死患者(AMI)体内胰岛素激活的一氧化氮合酶抑制剂(蛋白质)。

法律資訊

Sephadex is a registered trademark of Cytiva

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
W Windig et al.
Developments in biological standardization, 47, 169-177 (1981-01-01)
Poliovirus suspensions produced in microcarrier culture of primary monkey kidney cells and purified by column chromatography on DEAE-sephadex have been tested for the presence of DEAE-polysaccharides (in essence DEAE-dextran) by pyrolysis mass spectrometry. Semi-quantitative evaluation of the pyrolysis mass spectra
B Ullman et al.
Biochimica et biophysica acta, 403(2), 393-411 (1975-10-22)
A phosphoprotein phosphatase which is active against chemically phosphorylated protamine has been purified about 500-fold from bovine adrenal cortex. The enzyme has a pH optimum between 7.5 and 8.0, and has an apparent Km for phosphoprotamine of about 50 muM.
Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead

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