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Merck
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重要文件

SRP6522

Sigma-Aldrich

Ubiquitin-TAMRA-labeled (TMR-Ub)

≥95% (SDS-PAGE)

同義詞:

CEP80, HUBCEP80, RPS27A, Ribosomal Protein S27a, UBA80, UBB, UBCEP1, UBCEP80

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

化驗

≥95% (SDS-PAGE)

形狀

liquid

分子量

9.133 kDa

包裝

pkg of 50 μg

濃度

8 mg/mL

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

外觀

≥ 8 mg/mL in PBS.

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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