SRP6415

组织蛋白酶D 人

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• 同義詞: CLN10, CPSD, CTSD
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in HEK 293 cells
• 籤條: 6-His tagged (C-terminus)
• 化驗: ≥95% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: calculated mol wt 43.6 kDa; observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)
• 包裝: pkg of 10 μg
• 製造商／商標名: Sigma-Aldrich
• 儲存條件: dry at room temperature
• 技術: activity assay: suitable
• 雜質: <1 EU/μg endotoxin (LAL test)
• 適合性: suitable for molecular biology
• UniProt登錄號: P07339
• 應用: life science and biopharma
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... CTSD(1509)

描述

一般說明

Research area: Cell signalling. Cathepsin D belongs to the peptidase A1 family, an estrogenic-induced lysosomal protease. Cathepsin D can be cleaved into the following 2 chains: N-terminal light chain and C-terminal heavy chain, which is expressed in the aorta extracellular space (at the protein level). ). It is found in most mammalian cells and is located in thelysosomes.

This gene is mapped to human chromosome 11p15.5.

應用

Cathepsin D has been used in the protease digestion of haemoglobin.

生化／生理作用

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered an important biomarker for type 2 diabetes-dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.Cathepsin D is involved in various physiological processes like apoptosis, autophagy, and protein degradation. Cathepsin D plays an important role in the pathogenesis of Alzheimer’s disease, neuronal ceroid lipofuscinosis, and breast cancer.

外觀

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable