推薦產品
生物源
bovine spleen
品質等級
化驗
10—70% protein (biuret)
形狀
lyophilized powder
比活性
≥2.0 units/mg protein
分子量
~45 kDa
製造商／商標名
Sigma-Aldrich
技術
activity assay: suitable
顏色
dark brown
適合性
suitable for molecular biology
UniProt登錄號
應用
life science and biopharma
儲存溫度
−20°C
基因資訊
cow ... CTSD(282883)
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一般說明
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
應用
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
生化／生理作用
组织蛋白酶D是一种核内体-溶酶体天冬氨酸蛋白酶，与乳腺癌转移和阿尔茨海默病有关。 人们发现，在凋亡的人包皮成纤维细胞中，溶酶体释放组织蛋白酶D先于释放细胞色素c和膜电位丧失。 诱导凋亡后12至24小时，PC12细胞中的组织蛋白酶D水平升高。
單位定義
在pH 3.0、37 °C条件下，一个单位可在一分钟内使一毫升溶液的A280增加1.0（以血红蛋白为底物，对TCA可溶性产物进行测定（1 cm光程））。
外觀
含有柠檬酸盐缓冲液盐的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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