推薦產品
生物源
human
品質等級
重組細胞
expressed in E. coli (N-terminal FLAG® tagged)
化驗
≥95% (SDS-PAGE)
形狀
lyophilized powder
分子量
10 kDa
技術
mass spectrometry (MS): suitable
溶解度
0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless
0.05 M Tris pH 7.5: ≥10 mg/mL
儲存溫度
−20°C
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一般說明
泛素是一种高度保守的球蛋白，表面有赖氨酸。C末端包含Leu-Arg-Gly-Gly结构基序。
Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
應用
N端FLAG标签泛素可取代泛素，用于形成多聚泛素-蛋白结合物。FLAG标签可用于在抗-FLAG亲和柱上分离和富集蛋白结合物，以及在蛋白免疫印迹法中通过抗-FLAG抗体检测结合物。
人泛素可用作质谱的标准品。
人类泛素已用于酵母增殖细胞核抗原(PCNA)的单泛素化 和有丝分裂阻滞1（Dma1）缺陷的体外泛素化测定
生化／生理作用
泛素是存在于真核细胞组织中的小调节蛋白。外源性泛素可刺激许多细胞系中的细胞凋亡。 人乳头瘤病毒-16的E7蛋白可通过泛素-蛋白酶体途径刺激视网膜母细胞瘤蛋白的降解。
泛素通过其C末端甘氨酸残基的ε-氨基与蛋白质的赖氨酸残基相互作用。与泛素相互作用的蛋白质通过一个三步过程进行单泛素化（mono-ubiquitination）或多单泛素化（multi-mono-ubiquitination）。泛素化调节细胞内运输和蛋白质降解，并且该途径的失衡与疾病有关。
Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.
包裝
包装尺寸基于蛋白质含量
準備報告
人泛素可以以10.00-11.00mg/ml的浓度溶解在0.05M Tris-HCl中，得到澄清至轻微浑浊的无色溶液。
法律資訊
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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