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重要文件

SRP0153

Sigma-Aldrich

SMYD3 (full length) Active human

recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE)

同義詞:

KMT3E, Lysine N-methyltransferase 3E, SET and MYND domain containing 3, ZMYND1, Zinc finger MYND domain-containing protein 1

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in baculovirus infected insect cells

化驗

≥50% (SDS-PAGE)

形狀

aqueous solution

分子量

50 kDa

包裝

pkg of 20 μg

儲存條件

avoid repeated freeze/thaw cycles

濃度

>0.02 mg/mL

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... SMYD3(64754)

一般說明

Human SMYD3 (GenBank Accession No. BC031010), full length, with N-terminal DDDDK tag (FLAG), MW = 50 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml flag peptide, 20% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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分析證明 (COA)

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Ilham M Alshiraihi et al.
International journal of molecular sciences, 21(24) (2020-12-19)
SMYD3 is a lysine methyltransferase that regulates the expression of over 80 genes and is required for the uncontrolled proliferation of most breast, colorectal, and hepatocellular carcinomas. The elimination of SMYD3 restores normal expression patterns of these genes and halts

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