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Key Documents

P3397

Sigma-Aldrich

磷酸葡萄糖变位酶 来源于兔肌肉

ammonium sulfate suspension, ≥100 units/mg protein

同義詞:

PGM, α-D-葡萄糖-1,6-二磷酸酶, α-D-葡萄糖磷酸转移酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

rabbit muscle

品質等級

描述

ammonium sulfate suspension, >=100 units/mg protein

形狀

ammonium sulfate suspension

比活性

≥100 units/mg protein

儲存條件

(Tightly closed)

技術

activity assay: suitable

顏色

white to light yellow

異物活動

lactic dehydrogenase ≤0.5%
phosphoglucose isomerase ≤0.01%
pyruvate kinase ≤0.05%

運輸包裝

wet ice

儲存溫度

2-8°C

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一般說明

适合研究领域:细胞信号传导

磷酸葡萄糖变位酶(PGM),是一种保守酶,在动物、植物和微生物中大量存在,几乎分布于所有组织中。

應用

本品(磷酸葡萄糖变位酶)已用于:

  • 研究人类的糖原合成与磷酸葡萄糖变位酶缺乏
  • 通过检测磷酸葡萄糖变位酶的活性研究代谢调控
  • 试验A(参见文献),检测BL21(DE3)C43转化细胞中的糖原代谢密切相关酶
  • 蔗糖合酶(SuSy)和ADP葡萄糖焦磷酸化酶(AGPase)检测(3)
  • 在酶活性测定中结合烟酰胺腺嘌呤二核苷酸磷酸(NADP+)还原反应,测定来自蔗糖和无机磷酸(Pi)的葡萄糖-1-磷酸

生化/生理作用

磷酸葡萄糖变位酶(PGM)在糖酵解和糖异生中起着至关重要的作用。参与糖原和昆虫海藻糖的代谢。磷酸葡萄糖变位酶也参与植物和某些微生物的发育。葡萄糖-6-磷酸是核心代谢产物,因此PGM是蛋白质、脂质和核酸的代谢关键,对植物发育至关重要。磷酸葡萄糖变位酶催化葡萄糖-6-磷酸(G-6-P)和葡萄糖-1-磷酸(G-1-P)的相互转变。

單位定義

1 个单位将在 30°C、pH 7.4 下每分钟将 1.0 μmolα-D-葡萄糖 1-磷酸转化为 α-D-葡萄糖 6-磷酸。

外觀

(NH4)2SO4,pH 6.0,含 0.01%EDTA 的晶体混悬液

分析報告

缩二脲法测定蛋白

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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存取文件庫

Phosphoglucomutase; mechanism of action.
V JAGANNATHAN et al.
The Journal of biological chemistry, 179(2), 569-575 (1949-06-01)
H Sugie et al.
Neurology, 38(4), 602-605 (1988-04-01)
We report a 5-month-old boy with recurrent vomiting, lethargy, and poor weight gain. He had profound metabolic acidosis and nonketotic dicarboxylic aciduria. The serum and muscle carnitine levels were significantly low (60% and 10% of the control means, respectively), suggesting
Nora Alonso-Casajús et al.
Journal of bacteriology, 188(14), 5266-5272 (2006-07-04)
To understand the biological function of bacterial glycogen phosphorylase (GlgP), we have produced and characterized Escherichia coli cells with null or altered glgP expression. glgP deletion mutants (DeltaglgP) totally lacked glycogen phosphorylase activity, indicating that all the enzymatic activity is
M B Dworkin et al.
The Journal of biological chemistry, 262(35), 17038-17045 (1987-12-15)
When 32P-labeled phosphoenolpyruvate is injected into Xenopus laevis oocytes, a 50-60-kDa protein of subunit size Mr 29,000 is rapidly labeled, followed by a second (monomeric) protein of 66 kDa concomitant with the loss of label from the first protein. We
Sharita Timal et al.
Human molecular genetics, 21(19), 4151-4161 (2012-04-12)
Congenital disorders of glycosylation type I (CDG-I) form a growing group of recessive neurometabolic diseases. Identification of disease genes is compromised by the enormous heterogeneity in clinical symptoms and the large number of potential genes involved. Until now, gene identification

文章

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism through its actions on muscle, liver, and adipose tissue.

Instructions for working with enzymes supplied as ammonium sulfate suspensions

條款

This procedure may be used for all Phosphoglucomutase products except for Phosphoglucomutase, Catalog Number P4109.

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