SAE0215

Inorganic Pyrophosphatase from Escherichia coli

≥100 units/mL, buffered aqueous solution

• 分類程式碼代碼: 12352204

屬性

• 生物源: Escherichia coli
• 品質等級: 200
• 重組細胞: expressed in E. coli
• 等級: for molecular biology
• 描述: Recombinant, expressed in E.coli
• 化驗: ≥95% (size exclusion chromatography)
• 形狀: buffered aqueous solution
• 比活性: ≥100 units/mL
• 儲存期限: 2 yr at -20 °C ((retest))
• 分子量: 19.7 kDa
• 儲存條件: OK to freeze
• 濃度: ≥100 units/mL
• 顏色: colorless
• 最適pH: 9.0 (25 °C)
• pH值: 8.0 (25 °C)
• 溶解度: soluble; water: miscible
• 適合性: suitable for molecular biology
• UniProt登錄號: P0A7A9
• 應用: research use
• 異物活動: DNAse, none detected; RNAse, none detected; Nickase, none detected
• 運輸包裝: dry ice
• 儲存溫度: -10 to -25°C

描述

一般說明

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate hydrolysis. It plays an important role in energy metabolism by providing a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis. Nucleic acid synthesis would be energetically impossible in vivo if not coupled with the hydrolysis of pyrophosphate (PPi).

應用

This product is based on the native pyrophosphatase from E. coli, Uniprot No. P0A7A9. Pyrophosphatase in E. coli is a homohexameric protein containing 175 amino acids residues per subunit. This product is a recombinant protein expressed in E. coli and induced by IPTG. Each subunit has a MW of 19.7 kDa and theoretical pI of ~5. The protein activity is Mg2+ dependent and it is a relatively thermostable protein.

生化／生理作用

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme that catalyzes pyrophosphate to phosphate. It plays an important role in energy metabolism as it provides a thermodynamic pull for biosynthetic reactions, such as protein, RNA, and DNA synthesis.

特點和優勢

This product has a purity minimum of 95% (SEC-HPLC) and an activity minimum of 100 units/mL to enhance RNA yield during transcription.

單位定義

One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 9 at 25 °C. The reaction buffer used for determination of enzyme activity contains 50 mM Tris-HCl, pH 9.0.

外觀

The product is supplied as an aqueous solution containing 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% glycerol, titrated to pH 8 at 25 °C.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1