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一般說明
Starch is an insoluble starch substrate specific for α-amylase and not for other exo enzymes. α-amylase catalysis of starch azure solubilization is measured at 595 nm.
應用
Starch Azure has been used as a substrate
- in the screening of α-amylase activity in decontaminated malted flour at varying pH and temperature
- for monitoring the inhibitory activity of Physalis peruviana extracts
- for thermostable α-amylase extracted from deep sea and acidic soil
基底
Substrate for colorimetric determination of α-amylase.
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Despite being widely used, there is no standard protocol for α-amylase activity measurement with starch azure substrate. Boiling pre-treatment of starch azure suspension increased the reaction rate of hydrolysis catalysed by human salivary α-amylase (HSA) or porcine pancreatic α-amylase (PPA)
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