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PROTMEM

Millipore

ProteoPrep® 膜提取试剂盒

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

一般說明

ProteoPrep® 膜提取试剂盒试剂采用强力去垢剂来提高2D凝胶的蛋白上样率和分辨率。3和4型Protein Extraction Reagent 针对蛋白质组研究进行了优化。3和4型提取试剂是在等电位聚焦和2D电泳之前进行蛋白提取的最理想产品。 本试剂盒包含进行二硫键还原及烷基化所需的试剂。ProteoPrep® 膜提取试剂盒由Proteome Systems和Sigma的科研人员合作设计。

應用

ProteoPrep®膜提取试剂盒可从多种类型细胞制备高度富集的膜蛋白溶液。最终制得的蛋白溶液可用于2D凝胶电泳。

特點和優勢

  • 创新的去垢剂制备方法 - 提高了溶解度,可加载更多的蛋白质,提高了2D凝胶中低丰度蛋白的可见性。
  • 两种预混的增溶溶液 - 在提取前消除了干扰的非膜蛋白,提高了2D矩阵的整洁度。
  • 预测定的还原剂和烷化剂 - 试剂易于使用,可提供更高的IEF分辨率。

法律資訊

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 5 x 0.5SDS

  • C0356Protein Extraction Reagent Type 4 23 mLSDS

  • A3221Iodoacetamide, Single use vial of 56 mg 5 x 56SDS

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

閃點(°F)

186.8 °F - closed cup

閃點(°C)

86 °C - closed cup


分析證明 (COA)

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Lili Xu et al.
The Journal of general virology, 90(Pt 11), 2695-2703 (2009-07-25)
Raccoon dog is one of the suspected intermediate hosts of severe acute respiratory syndrome coronavirus (SARS-CoV). In this study, the angiotensin-converting enzyme 2 (ACE2) gene of raccoon dog (rdACE2) was cloned and sequenced. The amino acid sequence of rdACE2 has
Sreepriya Nair et al.
Electrophoresis, 32(24), 3621-3629 (2011-11-22)
Performing 2-DE of lipid-rich multilamellar membranes like myelin is a cumbersome task. However, for understanding its molecular organization and changes during diseases, identification of proteins of myelin is essential. Although the 2-D-proteomic approach of myelin has been employed to understand
Fang Chen et al.
Proteomics, 7(9), 1529-1539 (2007-04-05)
Plant plasma membrane (PM) proteins play important roles in signal transduction during defense response to an attacking pathogen. By using an improved method of PM protein preparation and PM-bound green fluorescent protein fusion protein as a visible marker, we conducted
Carla Cacciotto et al.
BMC microbiology, 10, 225-225 (2010-08-27)
Mycoplasmas are the simplest bacteria capable of autonomous replication. Their evolution proceeded from gram-positive bacteria, with the loss of many biosynthetic pathways and of the cell wall. In this work, the liposoluble protein complement of Mycoplasma agalactiae, a minimal bacterial
Haiqing Sheng et al.
Applied and environmental microbiology, 74(16), 5015-5022 (2008-06-17)
Escherichia coli O157:H7 causes hemorrhagic colitis and the life-threatening hemolytic-uremic syndrome in humans and transiently colonizes healthy cattle at the terminal rectal mucosa. To investigate the role of the O antigen in persistence and colonization in the animal host, we

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