推薦產品
用途
sufficient for 20 extractions
儲存溫度
−20°C
一般說明
Sigma的植物分级蛋白提取试剂盒(Plant Fractionated Protein Extraction Kit)是专为植物生命科学应用而设计的产品，能够从任意植物种类或组织中抽提亲水或疏水蛋白的定性样品，以帮助用户开展下游的蛋白组学应用。本方案无需任何超速离心步骤或含水多聚物的两相分离步骤(APTP)。本试剂盒包含五类试剂：一份植物特异性的蛋白酶抑制剂、一份用于抽提亲水蛋白质的专用配方试剂，一份增加了溶解能力以抽提更多疏水蛋白质的全新离液剂。以及还原剂三丁基膦(TBP)和烷化试剂碘乙酰胺。这些试剂能够增强等电聚焦和2-D凝胶电泳的分离效果。蛋白酶抑制剂是作用于丝氨酸、半胱氨酸、天冬氨酸、金属蛋白酶与氨肽酶等酶类的多种蛋白酶的广谱抑制剂混合物。含有4-（2-氨基乙基）苯磺酰氟（AEBSF）、抑氨肽酶素、抑肽素A、E-64、亮抑酶肽和1,10-菲咯啉。
在去除了多酚、鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提水溶性蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
在去除了多酚、鞣质等干扰物质之后，新鲜或冻存的植物组织经研磨后，用一个专用配方试剂重悬，来抽提水溶性蛋白。亲水蛋白顺序抽提完成后，加入所提供的离液剂以抽提疏水的膜蛋白。通过离心沉淀植物碎片，并收集蛋白抽提液。最后用户可获得定性的分级蛋白样品，用于下游蛋白组学分析。
套裝中的組件也可單獨購買
產品號碼
描述
SDS
- Plant Protein Extraction Reagent Type 1 1 bottle
- C0356Protein Extraction Reagent Type 4 1 bottleSDS
- P9599Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution 3 x 1SDS
- T7567Tributylphosphine solution, 200 mM (in N-methyl-2-pyrrolidinone), liquid 3 x 0.5SDS
- A3221Iodoacetamide, Single use vial of 56 mg 3 vial(s)SDS
訊號詞
Danger
危險分類
Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Repr. 1B - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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