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Key Documents

P7582

Sigma-Aldrich

氨基端 FLAG-BAP 融合蛋白

同義詞:

Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

形狀

liquid

品質等級

分子量

~49 kDa

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

氨基末端FLAG-BAP融合蛋白是大肠杆菌细菌碱性磷酸酶(BAP)的467个氨基酸的N末端FLAG融合蛋白,计算分子量为49.3kDa。根据电泳条件不同,N-末端FLAG-BAP融合蛋白在SDS-PAGE中迁移到45−55kDa条带。FLAG-BAP融合蛋白可用于生物技术应用,并可用于筛选重组融合蛋白。
氨基端 FLAG-BAP 融合蛋白是一种对照蛋白,用于确认抗 FLAG M1 和 M2 单克隆抗体在免疫印迹、免疫沉淀、ELISA、电子显微镜和 FACS 等应用中的功能完整性。

應用

氨基末端FLAG-BAP融合蛋白已用作标准品,用于骨桥蛋白的酶联免疫吸附测定(ELISA)以及Flag-Wnt8黑腹果蝇转录因子相互作用组(interactome)的蛋白质印迹定量分析。
请访问我们的 FLAG®应用门户网站,了解更多产品详情。

其他說明

对照蛋白

外觀

蛋白质供应形式为10mm Tris、120mm NaCl、
0.05 mM ZnCl2,50%甘油,pH 8.0。

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
BAP-fusion: A versatile molecular probe for biotechnology research
Biotechnology: Research, Technology and Applications, 327-345 (2008)
Daniela Castiglia et al.
Biotechnology for biofuels, 9, 154-154 (2016-07-28)
Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of

文章

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

相關內容

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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