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F4799

Sigma-Aldrich

3xFLAG™ Peptide

≥90% (HPLC/MS), lyophilized powder

同義詞:

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk肽, dykddddk肽

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About This Item

經驗公式（希爾表示法）:
C120H169N31O49S
分子量：:
2861.87
MDL號碼:
MFCD01863911
分類程式碼代碼:
12352202
NACRES:
NA.32

產品名稱

3X FLAG® 肽, lyophilized powder

化驗

≥90% (HPLC/MS)

品質等級

200

形狀

lyophilized powder

運輸包裝

wet ice

儲存溫度

2-8°C

相關類別

一般說明

3X FLAG肽是一种具有23个氨基酸残基的合成肽。在该肽中Asp-Tyr-Lys-Xaa-Xaa-Asp基序重复了三次。在C-末端的八个氨基酸组成了经典的FLAG序列(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)。
Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.

應用

用于从在溶液中的或结合至抗-FLAG® M2琼脂糖亲和凝胶（A2220）上琼脂糖上的抗-FLAG ®M2单克隆抗体（F1804）进行3X FLAG®融合蛋白的竞争性洗脱。通过亲和色谱法可实现这一目标。用于将3X FLAG融合蛋白从抗-FLAG® M2亲和凝胶上温和的洗脱。1X FLAG肽（F3290）将不会把3X FLAG融合蛋白从抗-FLAG®亲和凝胶上洗脱下来。


请访问我们的FLAG® 应用 门户网站，了解更多产品详情。

準備報告

如需制备储备溶液，可溶解于TBS（含有150 mM NaCl的50mM Tris-HCl，pH7.4）至5 mg/mL的浓度。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
SLCJ7677
SLCJ4916
SLCF2877
SLCG1967
SLCD1730

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Yannick Doyon et al.
Molecular and cellular biology, 24(5), 1884-1896 (2004-02-18)
The NuA4 histone acetyltransferase (HAT) multisubunit complex is responsible for acetylation of histone H4 and H2A N-terminal tails in yeast. Its catalytic component, Esa1, is essential for cell cycle progression, gene-specific regulation and has been implicated in DNA repair. Almost
Min Hwa Shin et al.
PLoS genetics, 12(2), e1005884-e1005884 (2016-03-02)
The inactivation of p53 creates a major challenge for inducing apoptosis in cancer cells. An attractive strategy is to identify and subsequently target the survival signals in p53 defective cancer cells. Here we uncover a RUNX2-mediated survival signal in p53
Andrew T Schiffmacher et al.
The Journal of cell biology, 215(5), 735-747 (2016-11-20)
During epithelial-to-mesenchymal transitions (EMTs), cells disassemble cadherin-based junctions to segregate from the epithelia. Chick premigratory cranial neural crest cells reduce Cadherin-6B (Cad6B) levels through several mechanisms, including proteolysis, to permit their EMT and migration. Serial processing of Cad6B by a
Monika Raab et al.
Nature communications, 8, 16001-16001 (2017-07-13)
Lymphocyte function-associated antigen 1 (LFA-1) affinity and avidity changes have been assumed to mediate adhesion to intercellular adhesion molecule-1 for T-cell conjugation to dendritic cells (DC). Although the T-cell receptor (TCR) and LFA-1 can generate intracellular signals, the immune cell
Radu Tusco et al.
Nature communications, 8(1), 1264-1264 (2017-11-04)
Selective autophagy is a catabolic process with which cellular material is specifically targeted for degradation by lysosomes. The function of selective autophagic degradation of self-components in the regulation of innate immunity is still unclear. Here we show that Drosophila Kenny
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文章

Anti-FLAG® M2 Magnetic Beads

Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.

相關內容

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein Expression

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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