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Key Documents

D1411

Sigma-Aldrich

d-脱硫生物素

≥98% (TLC)

同義詞:

5-甲基-2-氧代-4-咪唑烷己酸

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About This Item

經驗公式(希爾表示法):
C10H18N2O3
CAS號碼:
分子量::
214.26
EC號碼:
MDL號碼:
分類程式碼代碼:
12352200
eCl@ss:
34058011
PubChem物質ID:
NACRES:
NA.25

品質等級

化驗

≥98% (TLC)

形狀

powder

儲存溫度

2-8°C

SMILES 字串

C[C@@H]1NC(=O)N[C@@H]1CCCCCC(O)=O

InChI

1S/C10H18N2O3/c1-7-8(12-10(15)11-7)5-3-2-4-6-9(13)14/h7-8H,2-6H2,1H3,(H,13,14)(H2,11,12,15)/t7-,8+/m0/s1

InChI 密鑰

AUTOLBMXDDTRRT-JGVFFNPUSA-N

應用

d-脱硫生物素已用作洗脱缓冲液组分:
  • 在免疫共沉淀过程中从树脂上洗脱沉淀的蛋白质
  • 在蛋白质层析纯化过程中洗脱人 Cdc45-MCM-GINS (CMG) 解旋酶
  • 在层析纯化过程中,洗脱异二聚体驱动蛋白家族成员 3A/B (KIF3A/ B) 和驱动蛋白相关蛋白 3 (KAP3)-结合 APC armadillo (APCARM) 结构域

d-脱硫生物素已用于亲和层析和蛋白质层析。d-脱硫生物素可用于蛋白的标记、检测和分离。

生化/生理作用

脱硫生物素是一种不含硫的生物素衍生物,与生物素结合蛋白的结合强度较低,很容易被生物素移位。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Teegan A Delli-Bovi et al.
BMC biotechnology, 10, 73-73 (2010-10-13)
Biotin is an essential enzyme cofactor that acts as a CO2 carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step, insertion of sulfur
Bi-Huang Hu et al.
Analytical chemistry, 79(19), 7275-7285 (2007-08-24)
We describe a new method for encoded synthesis, efficient on-resin screening, and rapid unambiguous sequencing of combinatorial peptide libraries. An improved binary tag system for encoding peptide libraries during synthesis was designed to facilitate unequivocal assignment of isobaric residues by
Rocio Rodriguez-Melendez et al.
The Journal of nutrition, 133(5), 1259-1264 (2003-05-06)
In mammals, biotin serves as a coenzyme for carboxylases such as propionyl-CoA carboxylase. The expression of genes encoding interleukin-2 (IL-2) and IL-2 receptor (IL-2R)gamma also depends on biotin. Biotin metabolites are structurally similar to biotin, and their concentrations in tissues
M Sárdy et al.
Clinical chemistry, 45(12), 2142-2149 (1999-12-10)
Tissue transglutaminase (TGc) has recently been identified as the major, if not the sole, autoantigen of gluten-sensitive enteropathy (GSE). We developed and validated an ELISA based on the human recombinant antigen and compared it to existing serological tests for GSE
Sudha Purushothaman et al.
PloS one, 3(5), e2320-e2320 (2008-05-30)
Fatty acids are indispensable constituents of mycolic acids that impart toughness & permeability barrier to the cell envelope of M. tuberculosis. Biotin is an essential co-factor for acetyl-CoA carboxylase (ACC) the enzyme involved in the synthesis of malonyl-CoA, a committed

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