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Key Documents

F3040

Sigma-Aldrich

Anti-FLAG® M1小鼠单抗 小鼠抗

clone M1, purified immunoglobulin, buffered aqueous solution

同義詞:

抗 ddddk, 抗 dykddddk

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.32

生物源

(Bioreactor)

品質等級

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

M1, monoclonal

形狀

buffered aqueous solution

純化經由

using Protein A

物種活性

all

濃度

2-5 mg/mL

技術

western blot: 10 μg/mL

免疫原序列

DYKDDDDK

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

抗 FLAG M1 IgG2b 单克隆抗体与具有游离 N 端的 FLAG 标记物的蛋白结合。

纯化方法-蛋白A

免疫原

FLAG;肽序列 DYKDDDDK

應用

小鼠产生的单克隆抗-FLAG® M1 抗体适用于酵母、动物和大肠杆菌细胞的免疫沉淀、蛋白质印迹或EIA 。

成功使用本产品的应用和相关同行评议文章如下所述。
染色质免疫沉淀(1 篇文章)
免疫荧光(1 篇文章)

请访问我们的FLAG® 应用门户网站,了解更多产品详情。

生化/生理作用

当 FLAG 表位位于融合蛋白的游离氨基末端时与之结合。不与 Met-FLAG 融合蛋白结合,因此不会识别未处理的、细胞质表达的蛋白。结合具有Ca2+ 依赖性;该复合物在不存在钙离子的情况下解离。

外觀

溶于含 0.02% 叠氮化钠 (w/v) 的 10 mM 磷酸钠、150 mM 氯化钠溶液 (pH 7.4) 中。

準備報告

将抗体稀释至 10ug/mL,置于 0.05 M TBS、pH 7.4、含 1 mM 氯化钙的溶液中。

法律資訊

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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Andrew Best et al.
Nature communications, 5, 4760-4760 (2014-09-12)
Alternative splicing--the production of multiple messenger RNA isoforms from a single gene--is regulated in part by RNA binding proteins. While the RBPs transformer2 alpha (Tra2α) and Tra2β have both been implicated in the regulation of alternative splicing, their relative contributions
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Journal of immunology (Baltimore, Md. : 1950), 187(4), 1826-1834 (2011-07-12)
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BMC cancer, 12, 45-45 (2012-01-28)
Spy1 is a novel 'cyclin-like' activator of the G1/S transition capable of enhancing cell proliferation as well as inhibiting apoptosis. Spy1 protein levels are tightly regulated during normal mammary development and forced overexpression in mammary mouse models accelerates mammary tumorigenesis.

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