推薦產品
應用
该产品已用于植物组织中焦磷酸盐含量的测定。通过监测马铃薯块茎提取物中的NADH氧化，它也已用作焦磷酸盐含量的快速常规检查。
生化／生理作用
焦磷酸盐检测试剂盒将焦磷酸盐的产生与NADH的消耗偶联在一起。
包裝
包括技术公告。
成分
组成浓度
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
咪唑 × HCl, pH 7.4 45 mM
枸橼酸盐 5 mM
EDTA 0.10 mM
Mg2+, Mn2+, Co2+ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
牛血清白蛋白 5 mg/mL
糖稳定剂 5 mg/mL
PPi-PFK 0.5 单位/mL
醛缩酶 7.5 单位/mL
GDH 5 单位/mL
TPI 50 单位/mL
品質
一种包含果糖-6-磷酸激酶（焦磷酸依赖性）、醛缩酶、磷酸丙糖异构酶和α-甘油磷酸脱氢酶的偶联酶系统。
原則
每消耗一摩尔焦磷酸盐，两摩尔NADH被氧化为NAD+。在340 nm处通过分光光度法监测反应。
该试剂可用于检测10-100 nmole的焦磷酸盐。
该试剂可用于检测10-100 nmole的焦磷酸盐。
準備報告
将一瓶焦磷酸盐试剂的内容物溶于4.0 mL水中。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
客戶也查看了
Devin W Cochrane et al.
Plant science : an international journal of experimental plant biology, 265, 124-130 (2017-12-11)
Class 1 plant hemoglobins (phytoglobins) are upregulated during low-oxygen stress and participate in metabolism and cell signaling via modulation of the levels of nitric oxide (NO). We studied the effects of overexpression and knockdown of the class 1 phytoglobin gene
R Viola et al.
Plant physiology, 97(2), 638-643 (1991-10-01)
Pretreatment of discs excised from developing tubers of potato (Solanum tuberosum L.) with 10 millimolar sodium fluoride induced a transient increase in 3-phosphoglycerate content. This was followed by increases in triose-phosphate, fructose 1,6-bisphosphate and hexose-phosphate (glucose 6-phosphate + fructose 6-phosphate
Erick Strauss et al.
The Journal of biological chemistry, 277(50), 48205-48209 (2002-10-10)
Pantothenic acid (vitamin B(5)) is the natural precursor of coenzyme A (CoA), an essential cofactor in all organisms. The pantothenic acid antimetabolite N-pentylpantothenamide inhibits the growth of Escherichia coli with a minimum inhibitory concentration of 2 microm. In this study
A continuous spectrophotometric assay for argininosuccinate synthetase based on pyrophosphate formation.
W E O'Brien
Analytical biochemistry, 76(2), 423-430 (1976-12-01)
D A Smyth et al.
Plant physiology, 75(3), 862-864 (1984-07-01)
Pyrophosphate (PPi) was measured in pea (Pisum sativum L.) and corn (Zea mays L.) tissues by using an enzymic method based on PPi-dependent phosphofructokinase (PPi-PFK). Different organs of pea and corn seedlings were extracted to determine if PPi is present
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務