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Key Documents

F9252

Sigma-Aldrich

Folin & Ciocalteu 酚试剂

suitable for determination of total protein by Lowry method, 1.9-2.1 N

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About This Item

MDL號碼:
MFCD00132625
分類程式碼代碼:
12171500
NACRES:
NA.47

形狀

liquid

品質等級

200

濃度

1.9-2.1 N

顏色

clear yellow
faint yellow to very dark yellow, and Faint Green-Yellow to Very Dark Green-Yellow

pH值

<0.5 (20 °C)

密度

1.240 g/cm3 at 20 °C

適合性

suitable for determination of total protein by Lowry method

應用

diagnostic assay manufacturing
hematology
histology

儲存溫度

room temp

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相關類別

一般說明

Folin & Ciocalteu酚试剂最常用于Lowry法测定蛋白浓度。它还用于总酚类的定量。在该方法中，蛋白质用改进的缩二脲试剂（用酒石酸钠钾稳定的碱性铜溶液
）中的铜（II）进行预处理。酚试剂的加入可产生在550-750 nm间提高吸光值的色原。通常，在尖峰（750 nm）或肩峰（660 nm）处的吸光度用于定量1-100 mg/ml之间的蛋白浓度，而550nm处的吸光度用于定量更高的蛋白浓度。
在没有铜的情况下，颜色强度主要由蛋白中的酪氨酸和色氨酸含量决定，并在较小程度上由半胱氨酸和组氨酸决定。铜(II)对由酪氨酸、色氨酸或组氨酸而形成的颜色没有影响，但会由于半胱氨酸而减少颜色形成。
许多对原始检测流程的修改已得到发表，包括用于增强显色、测定不溶性蛋白含量以及过程的自动化。多种物质包括许多缓冲剂、螯合剂、去污剂和环状有机化合物都可对Lowry蛋白检测产生干扰。
Folin & Ciocalteu酚试剂可在色谱过程中用作喷雾剂。

應用

Folin & Ciocalteu酚试剂已用于样品中总酚量的预测。

其他說明

Folin & Ciocalteu酚试剂中不含酚。反而，试剂可与酚类和非酚类还原性物质反应形成可通过分光光度法检测的色原。

聯結

该产品也还作为试剂盒的一部分。

象形圖

Corrosion
GHS05

訊號詞

Danger

危險聲明

H290,H314

危險分類

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類（WGK）

WGK 2

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
BCCH0732
BCCG2765
SHBL2943
BCCD8944
BCCC7658

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存取文件庫

Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent.
Ainsworth EA and Gillespie KM
Nature Protocols, 2, 875-875 (2007)
David L Jones et al.
PloS one, 9(3), e90882-e90882 (2014-03-19)
Monitoring the properties of dissolved organic carbon (DOC) in soil water is frequently used to evaluate changes in soil quality and to explain shifts in freshwater ecosystem functioning. Using >700 individual soils (0-15 cm) collected from a 209,331 km(2) area
Han-Shin Kim et al.
Scientific reports, 6, 25318-25318 (2016-05-05)
Biofilm formation on biotic or abiotic surfaces has unwanted consequences in medical, clinical, and industrial settings. Treatments with antibiotics or biocides are often ineffective in eradicating biofilms. Promising alternatives to conventional agents are biofilm-inhibiting compounds regulating biofilm development without toxicity
Nutritional evaluation of some subtropical red and green seaweeds Part II. In vitro protein digestibility and amino acid profiles of protein concentrates.
Wong KH and Cheung PCK
Food Chemistry, 72, 11-17 (2001)
Lowry's method of protein estimation: some more insights.
S Sengupta et al.
The Journal of pharmacy and pharmacology, 45(1), 80-80 (1993-01-01)
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文章

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

條款

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Enzymatic Assay of Proteinase K (EC 3.4.21.64)

Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).

Enzymatic Assay of Protease Using Casein As a Substrate

To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.

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