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Merck
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重要文件

B6916

Supelco

Bradford试剂

for 0.1-1.4 mg/ml protein

同義詞:

考马斯染料结合蛋白检测,蛋白染料试剂

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About This Item

分類程式碼代碼:
12161500
NACRES:
NA.32

品質等級

形狀

solution

儲存溫度

2-8°C

相關類別

一般說明

Bradford检测是把考马斯亮蓝G-250加入至蛋白溶液中。考马斯蓝染料可与碱性和芳香族氨基酸结合,从而在蛋白质测定过程中引起吸光值的迁移。

應用

Bradford试剂已用于测定总蛋白的浓度。

特點和優勢

  • 该试剂为即用型。 无需混合或稀释。
  • 显色很快速。 仅需五分钟的孵育便能将样本在595 nm处进行读值。
  • 带有硫醇的还原性糖和还原性底物并不会干扰该试剂。
  • 试剂适用于微量(1-10 μg/ml)及标准(50-1400 μg/ml)的检测。
  • 可用于微孔板检测。
  • 低成本检测。

法律資訊

應用

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象形圖

Health hazardCorrosion

訊號詞

Warning

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2

標靶器官

Eyes,Central nervous system

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Rosenberg IM
Protein Analysis and Purification: Benchtop Techniques (2006)
Proteomic response of the biological control fungus Trichoderma atroviride to growth on the cell walls of Rhizoctonia solani.
Grinyer J et al.
Current Genetics, 47, 381-381 (2005)
Caleigh Mandel-Brehm et al.
Neurology, 93(5), e433-e444 (2019-07-05)
To identify molecular correlates of primary angiitis of the CNS (PACNS) through proteomic analysis of CSF from a biopsy-proven patient cohort. Using mass spectrometry, we quantitatively compared the CSF proteome of patients with biopsy-proven PACNS (n = 8) to CSF
Tracey Welham et al.
Journal of experimental botany, 60(12), 3353-3365 (2009-05-29)
Neutral/alkaline invertases are a subgroup, confined to plants and cyanobacteria, of a diverse family of enzymes. A family of seven closely-related genes, LjINV1-LjINV7, is described here and their expression in the model legume, Lotus japonicus, is examined. LjINV1 previously identified
Feng He et al.
BMC plant biology, 19(1), 552-552 (2019-12-14)
Understanding lignin biosynthesis and composition is of central importance for sustainable bioenergy and biomaterials production. Species of the genus Miscanthus have emerged as promising bioenergy crop due to their rapid growth and modest nutrient requirements. However, lignin polymerization in Miscanthus

文章

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

相關內容

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

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