推薦產品
應用
该试剂盒可在极少量样品中测量极低的蛋白浓度。准确测量蛋白浓度的范围，试管测定为0.5至30 μg/mL；96孔或 384孔平板测定则为1至20 μg/mL。
除了在溶液中测定和定量蛋白质外， QuantiPro™ BCA分析试剂盒还用于其他应用，包括测定与琼脂糖载体共价结合的蛋白质和吸附在多孔板上的蛋白质。 也用于测定各种固体载体上的官能团，如巯基、 N-羟基琥珀酰亚胺基羧酸盐、醛和酰肼。
特點和優勢
- 在广泛的蛋白浓度范围内均能维持高精确度
- 灵敏度高；蛋白浓度介于0.5 至 30 μg/ml之间时，具有线性响应
- 稳定的彩色复合物
- 降低了去垢剂的影响
原則
蛋白质在碱性条件下将Cu2+还原为Cu1+ ，形成二辛可宁酸:1+复合物，该复合物在562 nm处有最大吸光度。
儲存和穩定性
室温下储存试剂QA、QB和QC。不使用试剂 QC（4％ 五水硫酸铜（II）溶液12mL），仅将试剂QA和QB混合，置于密闭容器，可在室温下保存一周。如果本试剂盒遇冷或冷藏，则在试剂QA或试剂QB中可能会形成沉淀。要溶解沉淀，请在混合的同时将溶液温度缓慢升至室温。该溶液现在适合使用。
法律資訊
QuantiPro is a trademark of Sigma-Aldrich Co. LLC
僅套裝組件
產品號碼
描述
- Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL
套裝中的組件也可單獨購買
產品號碼
描述
SDS
- P0914Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1SDS
應用
產品號碼
描述
訂價
相關產品
產品號碼
描述
訂價
訊號詞
Danger
危險分類
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8A - Combustible corrosive hazardous materials
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
客戶也查看了
Eneida V Reis et al.
Parasitology international, 63(1), 120-126 (2013-10-24)
Vaults are ribonucleoproteins (13 MDa) highly conserved among lower and higher eukaryotes. Their association produces a complex composed of three proteins named Major Vault Protein (MVP), vault (PolyADP-ribose) polymerase (VPARP) and Telomerase-associated protein (TEP1), plus a small untranslated RNA. The
A new strategy for battling bacterial resistance: Turning potent, non-selective and potentially non-resistance-inducing biocides into selective ones
Ghanbar S, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine (2018)
Jingwei Zhang et al.
Acta biomaterialia, 10(7), 3254-3263 (2014-04-01)
The microporosity of calcium phosphate (CaP) ceramics has been shown to have an essential role in osteoinduction by CaP ceramics after ectopic implantation. Here we show that it is not the microporosity but the size of surface microstructural features that
PPAR-? agonist GL516 reduces oxidative stress and apoptosis occurrence in a rat astrocyte cell line
Neurochemistry International (2019)
Sook Hyun Chung et al.
Scientific reports, 6, 28993-28993 (2016-07-05)
Vascular changes and photoreceptor degeneration are features of age-related macular degeneration, diabetic retinopathy and macular telangiectasis. We have profiled the differential expression of microRNAs and analysed their target genes in transgenic mice in which induced Müller cell disruption results in
文章
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
相關內容
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.
我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.
聯絡技術服務