P6998

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• 同義詞: PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase
• 酶委員會編號: 2.7.11.11 (BRENDA, IUBMB)
• MDL號碼: MFCD07371063
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 重組細胞: expressed in baculovirus infected Sf9 cells
• 品質等級: 200
• 化驗: ≥80% (SDS-PAGE)
• 形狀: buffered aqueous glycerol solution
• 比活性: ≥10 nmol/min·mg
• 分子量: protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)
• UniProt登錄號: P22694
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PRKACB(5567)

描述

一般說明

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

The molecular weight of about 65kDa is confirmed by the SDS-PAGE gel. The product was generated by expressing recombinant full-length human PKAcβ2 with an additional N-terminal GST tag by baculovirus in Sf9 insect cells. The gene accession number is NM_002731. The resulting protein is 351aa cAMP-dependent protein kinase catalytic subunit beta isoform 2 (NP_002722), or according to UniProt, Isoform 1 (P22694-1).

應用

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 ^°C using a final concentration of 50 μM [³²P] ATP.

生化／生理作用

A catalytic subunit of cAMP-dependent protein kinase, the protein encoded by PRKACB catalyzes events downstream of GPCRs including cell cycle, differentiation and proliferation. When activated, this subunit acts on metabolic enzymes, ion channels and transcription factors such as CREB.

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

單位定義

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

外觀

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable