C8482

Protein Kinase A catalytic subunit human

>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

• 同義詞: Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
• MDL號碼: MFCD03458215
• 分類程式碼代碼: 41106305
• NACRES: NA.32

屬性

• 重組細胞: expressed in E. coli
• 品質等級: 200
• 化驗: >90% (SDS-PAGE)
• 形狀: buffered aqueous glycerol solution
• 比活性: >1000 units/mg protein
• 分子量: 43.5 kDa
• UniProt登錄號: P17612
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PRKACA(5566)

描述

生化／生理作用

Phosphorylates a spectrum of substrates, and regulates many cellular processes.

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

外觀

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

分析報告

One unit will transfer 1 nanomole of phosphate from ³²P-ATP to kemptide substrate per minute at pH 7.4 at 30 °C (measured by a radioactive filter-binding assay).
K_m (ATP) = 25 μM at 30 °C, pH 7.4. K_m (kemptide) = 42 μM (33 μg/ml) at 30 °C, pH 7.4.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable