跳轉至內容
Merck
全部照片(2)

Key Documents

View All Documentation

P3804

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

同義詞:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

登入查看組織和合約定價
全部照片(2)

About This Item

線性公式:
C6H4(NH2)2·2HCl
CAS號碼:
615-28-1
分子量：:
181.06
Beilstein:
3912045
EC號碼:
210-418-7
MDL號碼:
MFCD00012966
分類程式碼代碼:
12352204
PubChem物質ID:
24898457
NACRES:
NA.83

product name

邻苯二胺 二盐酸盐, tablet, 5 mg substrate per tablet

形狀

tablet

mp

258 °C (dec.) (lit.)

溶解度

water: 1 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI 密鑰

RIIWUGSYXOBDMC-UHFFFAOYSA-N

尋找類似的產品？ 前往 產品比較指南

相關類別

一般說明

邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。

應用

o-Phenylenediamine dihydrochloride has been used:
  • as a chromogenic substrate in indirect enzyme-linked immunosorbent assay (ELISA) to detect immunoglobin G (IgG) antibodies against certain recombinant antigens/proteins
  • as a streptavidin-horseradish peroxidase (HRP) substrate sandwich ELISA to analyze human interleukin (IL)-17A, IL-23, and IP-10
  • as a substrate in ELISA for determination of IgG-class antibodies to Mycobacterium bovis in serum

包裝

16 mg total tablet weight

重構

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

訊號詞

Warning

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

H G Marco et al.
Peptides, 21(9), 1313-1321 (2000-11-10)
We have isolated a peptide from extracts of sinus glands from a South African spiny lobster species, Jasus lalandii, by high-performance liquid chromatography (HPLC) and identified it as a putative molt-inhibiting hormone (MIH) by (i) an in vitro assay with
Joanne L Casey et al.
Methods in molecular biology (Clifton, N.J.), 421, 111-124 (2008-10-02)
Large repertoires of peptides displayed on bacteriophage have been extensively used to select for ligand-binding molecules. This is a relatively straightforward process involving several cycles of selection against target molecules, and the resulting ligands can be tailored to various applications.
Cassandra Koole et al.
The Journal of pharmacology and experimental therapeutics, 353(1), 52-63 (2015-01-30)
The glucagon-like peptide-1 receptor (GLP-1R) is a class B G protein-coupled receptor that has a critical role in the regulation of glucose homeostasis, principally through the regulation of insulin secretion. The receptor system is highly complex, able to be activated
Florian Krammer et al.
Clinical and vaccine immunology : CVI, 21(8), 1153-1163 (2014-06-20)
Emerging H7N9 influenza virus infections in Asia have once more spurred the development of effective prepandemic H7 vaccines. However, many vaccines based on avian influenza viruses--including H7--are poorly immunogenic, as measured by traditional correlates of protection. Here we reevaluated sera
Sarah L Rowland et al.
The Journal of experimental medicine, 211(10), 1977-1991 (2014-09-03)
Plasmacytoid dendritic cells (pDCs) have long been implicated in the pathogenesis of lupus. However, this conclusion has been largely based on a correlative link between the copious production of IFN-α/β by pDCs and the IFN-α/β "signature" often seen in human
查看所有相關論文

文章

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務