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重要文件

P1146

Sigma-Aldrich

酸性磷酸酶 来源于马铃薯

lyophilized powder, ≥3.0 units/mg solid

同義詞:

(Orthophosphoric-monoester phosphohydrolase acid optimum), 酸性磷酸酶 来源于马铃薯

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42010102
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

≥3.0 units/mg solid

分子量

69 kDa

運輸包裝

wet ice

儲存溫度

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI 密鑰

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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一般說明

马铃薯酸性磷酸酶是一种磷酸单酯酶,具有多种分子形式,分子量相近,但等电点不同。

應用

检测了马铃薯酸性磷酸酶在各种表面活性剂介质中的活性。 双(2-乙基己基)磺基琥珀酸钠,Brij 35和3mM的SDS抑制磷酸酶活性,而表面活性剂十六烷基三甲基溴化铵增强活性。
马铃薯酸性磷酸酶已用于评估新基因产物的潜在过敏性的研究中。 在一项研究中,它还用于在pH 7.0下从酪蛋白中去除8个磷酸基团。

生化/生理作用

酸性磷酸酶(APase)是一个酶家族,在pH 4-7最佳条件下,非特异性地催化磷酸单酯和酸酐水解产生无机磷酸盐。

單位定義

在pH 4.8 ,37℃ 下,一单位每分钟可水解 1.0 μmole的-硝基苯基磷酸酯。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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J Lalitha et al.
Biochemistry and molecular biology international, 41(4), 797-803 (1997-04-01)
The effect of three different classes of surfactants viz., anionic, cationic and neutral on catalytic activity of potato acid phosphatase (AcPase) was studied. Anionic surfactants bis(2-ethylhexyl) sodium sulfosuccinate (AOT) and sodium dodecyl sulfate (SDS) inhibited AcPase activity completely at 3
S I Tu et al.
Plant physiology, 88(1), 61-68 (1988-09-01)
Primary cell walls, free from cytoplasmic contamination were prepared from corn (Zea mays L.) roots and potato (Solanum tuberosum) tubers. After EDTA treatment, the bound acid phosphatase activities were measured in the presence of various multivalent cations. Under the conditions
P P Waymack et al.
Archives of biochemistry and biophysics, 288(2), 621-633 (1991-08-01)
An acid phosphatase (orthophosphoric monoester phosphohydrolase, acid optimum; EC 3.1.3.2) isoenzyme from wheat germ was purified 7000-fold to homogeneity. The effect of wheat germ sources and their relationship to the isoenzyme content and purification behavior of acid phosphatases was investigated.
Sishuo Cao et al.
Regulatory toxicology and pharmacology : RTP, 63(2), 181-187 (2012-04-17)
With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three
J B Vincent et al.
Trends in biochemical sciences, 17(3), 105-110 (1992-03-01)
Formation of phosphate esters by kinases has long been recognized as an important process in biochemistry, but the reverse reaction, hydrolysis of phosphate esters by phosphatases, has attracted less attention. Recent work suggests that phosphatases are as important as kinases

條款

Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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