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P3627

Sigma-Aldrich

酸性磷酸酶 来源于小麦胚芽

≥0.4 unit/mg solid

同義詞:

酸性磷酸酶 来源于小麦胚芽, (正磷酸单酯磷酸水解酶[酸最适])

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
eCl@ss:
42010102
NACRES:
NA.54

形狀

powder

比活性

≥0.4 unit/mg solid

分子量

58 kDa by gel filtration

儲存溫度

−20°C

InChI

1S/C6H10O2/c1-3-4-8-5-6(2)7/h1,6-7H,4-5H2,2H3

InChI 密鑰

GZCWLCBFPRFLKL-UHFFFAOYSA-N

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應用

酸性磷酸酶(APase)可非特异性地催化磷酸单酯和酸酐的水解以产生无机磷酸盐。它可用于研究磷酸盐的生产、转运和再循环,以及细胞的代谢和能量转导过程。 产品P3627来自于小麦胚芽,已被用于确定磷酸酶处理对3F3/2染色的影响

生化/生理作用

植物酸性磷酸酶(APase)位于细胞溶质、液泡和细胞壁中。植物酸性磷酸酶对于磷酸盐的获取非常重要,其可以调动土壤中的有机磷酸盐。 磷酸盐饥饿可诱导酸性磷酸酶的产生。 酸性磷酸酶起源于发芽过程中的糊粉和黄芩组织。 它可以水解植酸、ATP、蛋白质磷酸和核苷酸磷酸。 它可在一般代谢反应中起作用。 小麦胚胎APase由四种同工酶组成。 小麦胚芽APase的分子量为58 kDa(凝胶过滤),最适pH值为5.7,pH范围为4.0-7.0,最适温度为45°C。

品質

含有脂肪酶

單位定義

在pH 4.8,37 °C条件下,一个单位可在每分钟水解1.0 μmole 硝基苯磷酸盐。

準備報告

Prepared essentially by method of Singer, T.P., J. Biol.Chem., 174, 11 (1948).

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Zuzana Novotná et al.
FEBS letters, 554(1-2), 50-54 (2003-11-05)
Phospholipase D (PLD) forms the major family of phospholipases that was first discovered and cloned in plants. In this report we have shown, for the first time, that C2 phosphatidylinositol-4,5-bisphosphate (PIP2)-dependent PLD(s) from 5 day hypocotyls of Brassica oleracea associated
Yueming Liang et al.
Frontiers in microbiology, 11, 571209-571209 (2020-12-18)
phoD-harboring microorganisms facilitate mineralization of organic phosphorus (P), while their role in the regulation of soil P turnover under P-limited conditions in Pinus massoniana plantations is poorly understood. The aim of the present study was to investigate the effects of
Kinsley C French et al.
Biochemistry, 51(51), 10127-10136 (2012-12-12)
PAPf39, a 39-residue peptide fragment from human prostatic acidic phosphatase, has been shown to form amyloid fibrils in semen (SEVI), which increase HIV infectivity by up to 5 orders of magnitude. The sequence of the PAPf39 fibrillar core was identified
Ran Yanovich et al.
Clinical orthopaedics and related research, 471(4), 1365-1372 (2012-12-15)
With bone resorption rates greater than formation, stress fracture pathogenesis plausibly involves bone remodeling imbalance. If this is the case, one would anticipate serum levels of bone turnover markers would be higher in patients with stress fractures than in those

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Enzymatic Assay of Acid Phosphatase (EC 3.1.3.2)

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