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Merck
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重要文件

MAK540

Sigma-Aldrich

Beta-Hydroxybutyrate (Ketone Body) Assay Kit

sufficient for 200 colorimetric tests

同義詞:

Beta-Hydroxybutyrate Quantification Kit

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About This Item

分類程式碼代碼:
41116104
NACRES:
NA.25

檢測方法

colorimetric

相關疾病

endocrinological disorders, diabetes; gastrointestinal diseases; cancer

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Ketone bodies are produced by the liver and used peripherally as an energy source when blood glucose levels drop. The two main ketone bodies are beta-hydroxybutyrate (β-HB) and acetoacetate (AcAc), while acetone is the third abundant ketone body. Normally these two predominant ketone bodies are present in small amounts in the blood during fasting and prolonged exercise. In patients who have diabetes, alcohol or salicylate poisoning, hormone deficiency, childhood hypoglycemia and other acute disease states, large quantities of ketone bodies are found in the blood. The over-production and accumulation of ketone bodies in the blood (ketosis) can lead to pathological metabolic acidosis (ketoacidosis). Blood ketone testing methods that quantify beta-HB, the predominant ketone body in the blood (approximately 75%) have been used for diagnosing and monitoring treatment of ketoacidosis.

適合性

The Beta-Hydroxybutyrate Assay Kit offers a sensitive colorimetric assay for measuring βHB levels in biological samples.

原則

This assay is based on an enzyme coupled reaction of beta-HB, in which the product NADH can be specifically monitored by a NADH sensor. The signal can be measured by an absorbance microplate reader with the OD ratio at the wavelength of 570 nm to 610 nm. The detection limit of this assay kit is 4 µM beta-hydroxybutyrate in a 100 µL reaction volume.

象形圖

Corrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Skin Corr. 1B

儲存類別代碼

8A - Combustible corrosive hazardous materials

水污染物質分類(WGK)

WGK 3


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