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Key Documents

H9408

Sigma-Aldrich

β-羟丁酸脱氢酶 来源于勒氏假单胞菌

lyophilized powder, ≥200 units/mg protein

同義詞:

(R)-3-羟基丁酸:NAD+ 氧化还原酶, 3-HBDH

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bacterial (Pseudomonas lemoignei)

品質等級

形狀

lyophilized powder

比活性

≥200 units/mg protein

純化經由

chromatography

異物活動

lactic dehydrogenase ≤0.05%
malic dehydrogenase ≤0.1%

儲存溫度

−20°C

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一般說明

D-β-羟丁酸脱氢酶 (BDH) 是一种与膜结合的脂质需求酶。

應用

β-来源于勒氏假单胞菌 的羟丁酸脱氢酶已被用于偶联检测以证实乙酰乙酸酯的形成。
适用于通过Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974)中介绍的方法测定乙酰乙酸酯和D(-)-3-羟基丁酸

生化/生理作用

脱氮副球菌中,3-羟丁酸脱氢酶在胞内聚羟基丁酸酯和聚羟基戊酸酯的分解中起关键作用。
在哺乳动物系统中,β-羟丁酸脱氢酶位于线粒体内膜上,需要磷脂酰胆碱才能发挥活性。相反,在假单胞菌属中则为可溶性胞质酶,不需要磷脂别构活化剂。利用作为代谢能量源的酮体时,需要这种酶。其首先催化 3-羟基丁酸酯氧化成乙酰乙酸酯,这是酮体转化为柠檬酸的第一步,而后通过三羧酸循环(Krebs 循环)进一步参与代谢。

單位定義

在pH 7.8、温度37°C条件下,一单位每分钟可将1.0 μmol的 D-β-羟基丁酸氧化成乙酰乙酸盐。

外觀

含有蔗糖、β-NAD和Tris缓冲盐的冻干粉末

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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