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Key Documents

H8509

Sigma-Aldrich

β-羟丁酸脱氢酶 来源于球形红假单胞菌

Type V, lyophilized powder, 250-750 units/mg protein

同義詞:

(R)-3-羟基丁酸:NAD+ 氧化还原酶, 3-HBDH

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

種類

Type V

品質等級

形狀

lyophilized powder

比活性

250-750 units/mg protein

純化經由

chromatography

異物活動

lactate dehydrogenase ≤0.05%
malate dehydrogenase ≤0.1%

儲存溫度

−20°C

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應用

适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 D(-)-3-羟基丁酸酯。

生化/生理作用

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

單位定義

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

外觀

含 Tris 缓冲盐的冻干粉

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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