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Key Documents

M7133

Sigma-Aldrich

4-甲基伞形酮硫酸盐 钾盐

sulfatase substrate

同義詞:

4-甲基伞形基硫酸钾

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About This Item

經驗公式(希爾表示法):
C10H7KO6S
CAS號碼:
分子量::
294.32
Beilstein:
3803203
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32

品質等級

化驗

≥99% (HPLC)

形狀

powder

溶解度

water: 5 mg/mL, clear, colorless

螢光

λex 334 nm; λem 370 nm (pH 10.4)
λex 360 nm; λem 449 nm (Reaction products)

儲存溫度

−20°C

SMILES 字串

[K+].CC1=CC(=O)Oc2cc(OS([O-])(=O)=O)ccc12

InChI

1S/C10H8O6S.K/c1-6-4-10(11)15-9-5-7(2-3-8(6)9)16-17(12,13)14;/h2-5H,1H3,(H,12,13,14);/q;+1/p-1

InChI 密鑰

CSOCSPXOODWGLJ-UHFFFAOYSA-M

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一般說明

4-甲基伞形酮硫酸酯(4MUS)作为一种通用底物,是4-甲基伞形酮(4MU)的极性代谢物。

應用

4-甲基伞形酮硫酸酯钾盐已用作偶联荧光硫代转移酶测定的底物,以从3′-磷酸腺苷5′-磷酸盐再生3′-磷酸腺苷 5′-磷酸硫酸盐(PAPS),进而测定脑苷脂硫酸转移酶(CST)的酶活性。它还用作芳香基硫酸酯酶测定/4-甲基伞形酮硫酸酯(4-MUS)测定的底物,以测试蛋白磁珠复合体的酶活性。

生化/生理作用

4-甲基伞形酮硫酸酯(4MUS)能够进行脱硫,并参与4MU的无效循环。它可以用于测定多种硫酸酯酶的活性。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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S Ratna et al.
Hepatology (Baltimore, Md.), 17(5), 838-853 (1993-05-01)
Futile cycling between 4-methylumbelliferone and its sulfate and glucuronide conjugates was examined in the single-pass perfused rat liver preparation. The steady-state hepatic extraction ratio of 4-methylumbelliferone was found to be high (0.97) at a low input concentration of 0.005 mumol/L
Caroline J Dean et al.
Clinical chemistry, 52(4), 643-649 (2006-02-25)
Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and
Wei-Ti Chen et al.
Analytical biochemistry, 339(1), 54-60 (2005-03-16)
A sensitive fluorometric assay was developed for alcohol sulfotransferase (AST). This was the first continuous fluorometric assay reported for AST. It used 3'-phosphoadenosine 5'-phosphosulfate regenerated from 3-phosphoadenosine 5'-phosphate by a recombinant phenol sulfotransferase (PST) using 4-methylumbelliferyl sulfate as the sulfuryl
Lauren J McAllister et al.
PloS one, 7(2), e32385-e32385 (2012-03-01)
Streptococcus pneumoniae (the pneumococcus) is a formidable human pathogen, responsible for massive global morbidity and mortality. The ability to utilize carbohydrates in a variety of host niches appears to be integral to pneumococcal pathogenesis. In this study we investigated a
M Chiba et al.
The Journal of pharmacology and experimental therapeutics, 266(2), 492-499 (1993-08-01)
4-Methylumbelliferyl sulfate (4MUS), a polar metabolite of 4-methylumbelliferone (4MU), is known to undergo desulfation and participate in futile cycling with 4MU. Unusual parabolic or increasing profiles of the steady-state extraction ratio (Ess) of 4MUS with respect to concentration in rat

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