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Key Documents

L3755

Sigma-Aldrich

脂多糖 来源于大肠杆菌 026:B6

purified by trichloroacetic acid extraction

同義詞:

LPS

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About This Item

EC號碼:
MDL號碼:
分類程式碼代碼:
12352201
NACRES:
NA.25

生物源

Escherichia coli (O26:B6)

品質等級

形狀

lyophilized powder

純化經由

trichloroacetic acid extraction

雜質

1-10% Protein (Lowry)

顏色

white to yellow

溶解度

water: soluble

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

该产品是从大肠杆菌O26:B6血清型中提取的三氯乙酸。来源菌株为ATCC12795。来自血清型026:B6的LPS是来自大肠杆菌的唯一在SDS-PAGE上表现出短链长行为的LPS。该LPS的短链长度更接近于突变型粗菌株LPS的短链长度。

應用

脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征成分。LPS及其脂质A部分通过Toll样受体4(TLR4)刺激先天免疫系统的细胞,TLR4是Toll样受体蛋白家族的成员,该家族可识别常见的病原体相关分子模式(PAMP)。

生化/生理作用

脂多糖(LPS)位于膜的外层,并且在非包封的菌株中,其暴露于细胞表面。它们有助于外膜的完整性,并可保护细胞免受胆汁盐和亲脂性抗生素的作用。

準備報告

该产品可溶于水(5mg / ml)或细胞培养基(1mg / ml),产生浑浊的淡黄色溶液。当涡旋并升温至70-80 oC后,在盐水中可获得更高浓度的溶液(20mg/ml),但仍然是浑浊的溶液。脂多糖是一种在各种溶剂中都形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。有机溶剂无法使溶液变澄清。甲醇会产生含有漂浮物的混浊悬浮液,而水会产生均匀的混浊溶液。

其他說明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形圖

Skull and crossbones

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 2 Oral

儲存類別代碼

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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存取文件庫

Guohui Cai et al.
Scientific reports, 6, 21097-21097 (2016-02-13)
Early life diet can critically program hypothalamic-pituitary-adrenal (HPA) axis function. We have previously shown rats that are overfed as neonates have exacerbated pro-inflammatory responses to immune challenge with lipopolysaccharide (LPS), in part by altering HPA axis responses, but how this
Rupali Sharma et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 71, 94-104 (2018-08-31)
Puberty is a critical developmental period that is characterized by significant brain development. Exposure to stress during this time can alter brain functioning setting the stage for long-lasting behavioural outcomes. The objective of this study was to investigate age and
Rupali Sharma et al.
Journal of immunology (Baltimore, Md. : 1950), 202(7), 2131-2140 (2019-02-10)
Puberty is a critical period of development marked by sexual, immune, and neural maturation. Exposure to stress during this period can lead to enduring changes in brain functioning and in behavior; however, the underlying mechanisms and the programming effects of
Rose B Joachim et al.
Molecular systems biology, 14(5), e7998-e7998 (2018-05-19)
Attempts to develop drugs that address sepsis based on leads developed in animal models have failed. We sought to identify leads based on human data by exploiting a natural experiment: the relative resistance of children to mortality from severe infections
Rose Joachim et al.
Scientific reports, 7(1), 16541-16541 (2017-12-01)
Sepsis is a common and deadly syndrome in which a dysregulated host response to infection causes organ failure and death. The current lack of treatment options suggests that a new approach to studying sepsis is needed. Pre-pubertal children show a

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