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Key Documents

L0418

Sigma-Aldrich

LB Agar Carbenicillin - 100, Plates

pre-poured agar plates with 100 μg/ml carbenicillin

同義詞:

carbenicillin agar plates

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About This Item

分類程式碼代碼:
41121800

等級

for molecular biology

品質等級

無菌

sterile; autoclaved

形狀

plates (10 cm)
plates (10cm, 20-25ml each)

存貨情形

available only in USA

容量

20-25 mL

儲存溫度

2-8°C

適合性

selective for Escherichia coli
selective for coliforms

一般說明

Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

應用

LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.

特點和優勢

Miller′s LB Agar plates provide:
  • Convenient, ready-to-use 10cm format
  • Standard formulation plus antibiotic

成分

15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin

其他說明

The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
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Biotechniques, 30(5), 963-965 (2001)

文章

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have enabled expression and isolation of recombinant proteins in large scale.

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