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G4155

Sigma-Aldrich

β 半乳糖苷酶 来源于大肠杆菌

aqueous glycerol suspension, ≥500 units/mg protein (biuret)

同義詞:

β-D-半乳糖苷半乳糖水解酶, 乳糖酶

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About This Item

CAS號碼:
酶委員會編號:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

aqueous glycerol suspension

品質等級

比活性

≥500 units/mg protein (biuret)

分子量

465 kDa

儲存溫度

−20°C

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相關類別

應用

β-半乳糖苷酶用于咪唑并吡咯烷糖的酶促合成。

生化/生理作用

β-半乳糖苷酶可将乳糖切割成单糖成分(葡萄糖和半乳糖)。它还在反馈环中催化葡萄糖转糖基化为β-半乳糖苷酶的诱导物 - 异乳糖。

物理性質

四聚体的分子量为465 kDa(每个亚基116.3 kDa)

單位定義

在 37°C、pH 7.3 条件下,1 个单元每分钟水解 1.0 μmol 邻硝基苯基 β-D-半乳糖苷,生成邻硝基苯酚和 D-半乳糖。

外觀

混悬于 50% 甘油、5 mM Tris 缓冲盐、5 mM 氯化镁、0.5 mM DTT、0.5 mM 巯基乙醇中,pH 7.4。

其他說明

如果该酶被冷冻,可能发生酶活性丧失。这种酶在 50% 甘油溶液中提供,如果在-20°C 下储存,不会冷冻。不建议将此酶制剂保存在超冷冰箱中 (-60 至-100° C)。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Théophile Tschamber et al.
Bioorganic & medicinal chemistry, 11(17), 3559-3568 (2003-08-07)
The syntheses of four glyco-imidazoles, which are pentose-derivatives belonging to the D-series, as well as the syntheses of their L-enantiomers, are reported. Starting from the known linear xylo, lyxo, arabino, and ribo imidazolo-pentoses in both the L- and the D-series
Olga Ermakova et al.
Brain sciences, 11(6) (2021-07-03)
Acquisition of detailed anatomical and molecular knowledge from intact biological samples while preserving their native three-dimensional structure is still a challenging issue for imaging studies aiming to unravel a system's functions. Three-dimensional micro-CT X-ray imaging with a high spatial resolution
Christophe Laumonnerie et al.
Development (Cambridge, England), 141(3), 594-603 (2014-01-23)
The establishment of anatomically stereotyped axonal projections is fundamental to neuronal function. While most neurons project their axons within the central nervous system (CNS), only axons of centrally born motoneurons and peripherally born sensory neurons link the CNS and peripheral
H Morreau et al.
The Journal of biological chemistry, 264(34), 20655-20663 (1989-12-05)
We have isolated two cDNAs encoding human lysosomal beta-galactosidase, the enzyme deficient in GM1-gangliosidosis and Morquio B syndrome, and a beta-galactosidase-related protein. In total RNA from normal fibroblasts a major mRNA of about 2.5 kilobases (kb) is recognized by cDNA
A Hinek
Biological chemistry, 377(7-8), 471-480 (1996-07-01)
The 67-kDa protein identical to the enzymatically inactive spliced variant of beta-galactosidase is a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. It recognizes several non-identical hydrophobic

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