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E6654

Millipore

EZview 红色c-Myc亲和凝胶抗体

同義詞:

Anti-c-Myc affinity resin, Anti-c-Myc agarose beads

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.56

儲存期限

1 yr at 2‑8 °C

品質等級

分析物化學類別

proteins (c-Myc)

技術

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

~0.05 mg/mL binding capacity

儲存溫度

2-8°C

一般說明

EZ view 红色c-Myc亲和凝胶抗体是一种树脂,由抗c-Myc兔抗与4%琼脂糖珠共价结合而形成。亲和凝胶用于将c-Myc融合蛋白与样品(如细胞裂解物和组织)结合,以便在免疫沉淀测定准备过程中纯化c-Myc标签蛋白。红色染料可增强能见度,从而获得更有效的结果。
琼脂糖珠与大肠杆菌(E. coli)和哺乳动物细胞的N末端、Met-N末端和C末端c-Myc融合蛋白结合。

應用

EZview® 红色c-Myc亲和凝胶抗体适用于:
  • 类泛素化修饰检测
  • 对大肠杆菌(E. coli)或哺乳动物细胞的N-或C-末端c-Myc标签融合蛋白的亲和捕获(免疫沉淀)。
在小规模亲和捕获中,如免疫沉淀,难以在微量离心管中看到亲和基质。 在移液时意外吸取树脂,会导致结果产生数量差异。EZview 红色亲和凝胶可大大降低沉淀损失的风险。 EZview树脂的性能与传统无色亲和凝胶相同,但更易于区分沉淀和上清液。 这将获得更准确的数据,因为蛋白质损失减少。

特點和優勢

  • 提高能见度 - 红色指示可降低意外吸取沉淀的风险
  • 通过减少意外损失,提高目标蛋白质的回收率
  • 更高的重现性 - 更稳定的得率

其他說明

亲和凝胶含有与琼脂糖珠共价结合的亲和纯化c-Myc兔多克隆抗体。 c-Myc标签对应于人c-Myc蛋白的氨基酸残基410-419(EQKLISEEDL)。

外觀

溶剂为PBS的1:1(v/v)悬浮液,含有50%甘油和0.0015%Kathon CG/IPCII作为抗菌防腐剂

法律資訊

EZview is a trademark of Sigma-Aldrich Co. LLC
SabreLite is a registered trademark of Pelican Products, Inc.

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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