生物源
rat
品質等級
共軛
agarose conjugate
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
3F10, monoclonal
形狀
slurry
包裝
pkg of 1 mL (settled resin volume)
製造商/商標名
Roche
同型
IgG1
表位序列
YPYDVPDYA
容量
2-8 nmol/mL binding capacity
儲存溫度
2-8°C
一般說明
抗-HA(3F10)识别9-氨基酸序列,YPYDVPDYA,这一序列来自人类流感血凝素(HA)蛋白。无论位置在哪儿(N末端、C末端或是中间),该抗原决定簇都可被来自哺乳动物、细菌和酵母表达载体的融合蛋白识别。
特異性
抗-HA(3F10)识别9-氨基酸序列,YPYDVPDYA,这一序列来自人类流感血凝素(HA)蛋白。无论位置在哪儿(N末端,C末端或是中间),该抗原决定簇都可以被来自哺乳动物,细菌和酵母表达载体的融合蛋白识别。
免疫原
98-106氨基酸来自人类流感病毒血凝素蛋白
應用
在以下实验中使用抗HA亲和基质:
- 来自哺乳动物,细菌和酵母细胞提取物的HA标记蛋白免疫沉淀
- 亲和柱纯化粗蛋白提取HA标记蛋白
在免疫沉淀或纯化之后,感兴趣的标记蛋白可以用于下列分析:
- 使用抗-HA抗体Western印迹
- 银染色(或相似的蛋白质染色)
- 免疫共沉淀。
特點和優勢
- 获得高特异性和抗-HA高亲和还原交叉反应性。
- 使用非变性条件,洗脱目的HA标记蛋白。
- 纯化稀有HA标记表达蛋白。
- 对YPYDVPDYA有高特异性。
- 适合纯化含有HA抗原决定簇的蛋白,可以是N端,C端或中间融合。
- 适用于哺乳动物,细菌和酵母表达体系。
品質
每一个批次的抗-HA亲和基质都经过HA标记蛋白纯化能力测试。这些蛋白是来自粗哺乳动物提取液感染细胞稳定表达蛋白。使用抗HA,高亲和抗体,western印迹法进行免疫沉淀。
外觀
PBS中1ml固定树脂抗-HA亲和基质,含有0.09%叠氮化钠(w/v);2ml重悬液等于1ml床柱。一个塑料柱包含顶端和底部的盖子。
分析報告
使用含有HA标记细菌碱性磷酸酶的全细胞细菌提取液能够获得9nmol纯化蛋白/ml亲和基质。
其他說明
仅用于生命科学研究。不可用于诊断。
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
does not flash
閃點(°C)
does not flash
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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Anti-HA Affinity Matrix Protocol & Troubleshooting
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