LL24

90073001, human lung, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: LL24, 90073001
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: 2n = 46; diploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human diploid lung

細胞系描述

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

應用

Tumourigenicity studies

培養基

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, a further 20 population doublings expected.

其他說明

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