HKT-1097

98061003, hamster kidney, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• product name: HKT-1097, 98061003
• 生物源: hamster kidney
• 增長模式: Adherent
• 染色體組型: Modal no. 44
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: EGF
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Syrian Hamster kidney

細胞系描述

The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.

DNA分析

Not specified

培養基

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Form a multipolar, loosely packed monolayer.

其他說明

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