IEC 18

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88011801, rat intestine, Epithelial

• 分類程式碼代碼: 41106514

屬性

• 生物源: rat intestine
• 包裝: tube of 5 μg 88011801-DNA-5UG; pkg of vial of cells 88011801-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Normal rat ileum

細胞系描述

Derived from normal epithelial cells of the rat ileum. Cells should undergo at least 20 population doublings. The line was established using the same techniques as for IEC6 (Sigma Catalogue number. 88071401). IEC 18 cells grow in small islands and do not reach confluency.

應用

Small bowel biology

培養基

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C

其他說明

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