HRT-18

86040306, human colon, Epithelial-like

• 分類程式碼代碼: 41106514
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 相關疾病: cancer

屬性

• 產品名稱: HRT-18, 86040306
• 生物源: human colon
• 增長模式: Adherent
• 染色體組型: 2n = 46
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human rectum adenocarcinoma

細胞系描述

Susceptible to bovine enteric coronaviruses. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin see.

應用

Virus studies: bovine enteric coronaviruses

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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