推薦產品
product name
HCT 116 Cell Line human, 91091005, colon carcinoma
生物源
human colon
增長模式
Adherent
染色體組型
Modal no. 45
形態學
Epithelial-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human colon carcinoma
細胞系描述
One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts.
應用
HCT 116 Cell Line has been used to:
- study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA)
- study the effects of different amounts of transferrin within the polyethyleneimine (PEI)-small interfering RNA (siRNA) complexes on the uptake of fluorescently-labelled siRNA
- determine the effect of PTS (polyamine transport system) in the selective uptake of Quilamine HQ1-44, an iron chelator
Tumourigenicity studies
DNA分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22
CSF1PO: 7,10
D13S317: 10,12
D16S539: 11,13
D5S818: 10,11
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22
培養基
McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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文章
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
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