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Key Documents

HT29-MTX-E12

12040401, human colon, Epithelial

同義詞:

HT-29-MTX-E12 Cells, HT29, HT29 Cells, HT29-MTX, HT29-MTX Cells, MTX-E12 Cells

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About This Item

分類程式碼代碼:
41106514

product name

HT29-MTX-E12, 12040401, from human colon

生物源

human colon

增長模式

Adherent

染色體組型

Not specified

形態學

Epithelial

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption

細胞系描述

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and productio

DNA分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17

培養基

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 7.5-10% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

其他說明

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分析證明 (COA)

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