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Merck
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C6788

Sigma-Aldrich

4-氯-1-萘酚

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

同義詞:

4-CN, 4-chloronaphthalen-1-ol

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About This Item

線性公式:
ClC10H6OH
CAS號碼:
分子量::
178.61
Beilstein:
509750
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

4-氯-1-萘酚, tablet

形狀

tablet

mp

118-121 °C (lit.)

溶解度

methanol: 10 mL, clear, colorless

儲存溫度

−20°C

SMILES 字串

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

InChI 密鑰

LVSPDZAGCBEQAV-UHFFFAOYSA-N

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相關類別

特異性

4-氯-1-萘酚是一种适合用于免疫印迹程序的过氧化物酶底物。该底物产生的不溶性最终产物为蓝色,可目视观察。

應用

4-氯-1-萘酚已被用作酶联免疫斑点(ELISpot)试验的底物,用于检测斑点。它还被用作沉淀生色底物,用于免疫印迹后抗原−抗体反应性条带的成像。

數量

每片含 30 mg 基质。

重構

将 1 片片剂溶于 10 mL 甲醇中。向 10 mL 三乙醇胺缓冲盐水 (pH 7.5) 中加入 2 mL 甲醇贮备液。临用前加入 5μl 新鲜 30% 过氧化氢(Sigma 产品编号:H-1009)。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Gloves


分析證明 (COA)

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Neospora caninum ranges among the major causes of infectious abortion in cattle worldwide. The present study was designed to improve the serodiagnostic tools by complementing a conventional ELISA with a highly sensitive and species-specific N. caninum immunoblot. To evaluate this
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C F Frey et al.
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文章

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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