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P8849

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic, aminopeptidases and thermolysin-like activities, for use in purification of Histidine-tagged proteins, DMSO solution

同義詞:

Protease inhibitor solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352200
NACRES:
NA.77

product name

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

生物源

synthetic

品質等級

形狀

DMSO solution

儲存溫度

−20°C

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一般說明

蛋白酶抑制剂的DMSO溶液专用于纯化组氨酸标签蛋白。

溶液中混有多种抑制剂成分,可特异性抑制丝氨酸、半胱氨酸、天冬氨酸、嗜热菌蛋白酶样蛋白酶和氨基肽酶。

特異性

抑制丝氨酸,半胱氨酸,天冬氨酸,和嗜热菌蛋白酶样蛋白酶和氨基肽酶。

應用

混合液经过专门的优化和验证,适合分离组氨酸标签(His-tag)蛋白质,特意去除螯合剂后尤其适合用于固定化金属亲和层析(IMAC)分离。

推荐用量:1 mL溶液抑制20g大肠杆菌细胞或10g杆状病毒感染草地夜蛾蛹卵巢细胞得到的100ml细胞裂解液中的蛋白酶活性。

特點和優勢

专门为组氨酸标签蛋白纯化而配制。

靶向多种蛋白酶,具有全面的蛋白酶抑制活性。

不加螯合剂,适合IMAC应用。

方便的包装选择。

成分

AEBSF
乌苯美司
E-64
Pepstatin A
磷阿米酮

數量

推荐采用1mL抑制从20gEscherichia coli或10g杆状病毒感染 Spodoptera frugiperda蛹卵巢细胞提取的总体积100ml的蛋白酶。

该蛋白酶抑制剂混合物已针对组蛋白标记蛋白进行了优化和测试

準備報告

该产品在DMSO中是澄清溶液。 推荐1mL溶液可以抑制20gE. Coli细胞或10g细胞或10g 杆状病毒感染细胞得到的100ml细胞裂解液中的蛋白酶活性。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

185.0 °F - closed cup

閃點(°C)

85 °C - closed cup


分析證明 (COA)

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Neuroscience, 256, 456-466 (2013-10-26)
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