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539134

Millipore

Protease Inhibitor Cocktail III

EDTA-Free, liquid, for the inhibition of aspartic, cysteine, and serine proteases and aminopeptidases in mammalian cells and tissues.

同義詞:

无EDTA蛋白酶抑制剂复合物, 蛋白酶抑制剂混合物III

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.77

product name

蛋白酶抑制剂混合物套装III,无EDTA, Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

形狀

liquid

製造商/商標名

Calbiochem®

儲存條件

OK to freeze

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

注意:以1 x 1 ml或一组5 x 1 ml形式提供。
该蛋白酶抑制剂混合物是由六种蛋白酶抑制剂组成的特殊混合物,具有广泛的特异性,可抑制天冬氨酸、半胱氨酸和丝氨酸蛋白酶以及氨基肽酶。建议将该混合物与哺乳动物细胞和组织提取物一起使用,但也适用于细菌提取物。它以单个试剂瓶或一组5个试剂瓶的形式提供。每个小瓶包含1 ml蛋白酶抑制剂混合物,该混合物溶于含有少量水的DMSO中。

特異性

广泛抑制丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶以及氨基肽酶。

應用

蛋白酶抑制剂混合物套装III(无EDTA)的应用实例:
  • 作为组分加入CelLytic MT细胞裂解试剂或裂解缓冲液以均质化肾脏类器官用于免疫印迹分析
  • 加入均质缓冲液以均质化小鼠股四头肌组织
  • 加入裂解缓冲液以提取蛋白用于免疫印迹

生化/生理作用

蛋白酶抑制剂混合物和特异性蛋白酶抑制剂在科研人员进行的各种蛋白质组研究中发挥重要作用。研究包括蛋白质表达和表征、生物标志物发现、翻译后修饰鉴定、蛋白表达谱分析和蛋白定量测定。蛋白酶抑制剂混合物或特异性蛋白酶抑制剂确保蛋白保留完整性,以备准确的下游分析和全面的样品鉴定。

特點和優勢

  • 不含EDTA:推荐用于固定化金属亲和色谱(IMAC)。
  • 多用:适合处理细菌提取物、哺乳动物细胞提取物和组织提取物。
  • 快速:片剂溶解无需等待。
  • 经济:即用形式减少浪费。
  • 高效:1 ml可处理约20 g组织。
  • 全面:包含可抑制天冬氨酸、半胱氨酸、丝氨酸蛋白酶和氨基肽酶的6种蛋白酶抑制剂。

警告

毒性:刺激性(B)

重構

初次融化后,等分并冷冻(-20°C)。

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Eye Irrit. 2 - Skin Irrit. 2

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

188.6 °F - (refers to pure substance)

閃點(°C)

87 °C - (refers to pure substance)


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The Journal of cell biology, 219(4) (2020-04-25)
Epithelial cell physiology critically depends on the asymmetric distribution of channels and transporters. However, the mechanisms targeting membrane proteins to the apical surface are still poorly understood. Here, we performed a visual forward genetic screen in the zebrafish intestine and
Olga Vasiljeva et al.
Scientific reports, 10(1), 5894-5894 (2020-04-05)
Proteases have been implicated in the development of many pathological conditions, including cancer. Detection of protease activity in diseased tissues could therefore be useful for diagnosis, prognosis, and the development of novel therapeutic approaches. Due to tight post-translational regulation, determination
Cao Chen et al.
Medical microbiology and immunology, 209(1), 81-94 (2019-11-14)
Activation of complement system in central nervous system (CNS) of the patients suffering from prion diseases or animal models infected with prion agents experimentally is reported repeatedly, but which pathways are involved in the complement system during prion infection is
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Journal of experimental botany, 72(7), 2514-2524 (2020-12-15)
Phosphoenolpyruvate carboxykinase (PEPCK) plays a crucial role in gluconeogenesis. In this work, we analyze the proteolysis of Arabidopsis thaliana PEPCK1 (AthPEPCK1) in germinating seedlings. We found that the amount of AthPEPCK1 protein peaks at 24-48 h post-imbibition. Concomitantly, we observed
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American journal of physiology. Renal physiology, 318(4), F956-F970 (2020-02-25)
Aquaporin-2 (AQP2) is a vasopressin-regulated water channel protein responsible for osmotic water reabsorption by kidney collecting ducts. In response to vasopressin, AQP2 traffics from intracellular vesicles to the apical plasma membrane of collecting duct principal cells, where it increases water

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