推薦產品
形狀
lyophilized
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
desiccated (hygroscopic)
運輸包裝
wet ice
儲存溫度
−20°C
相關類別
一般說明
蛋白酶抑制剂混合液Set I A 是一种专门配制的混合液，含有可抑制各种蛋白酶和酯酶的五种不同蛋白酶抑制剂。
特異性
抑制多种蛋白酶和酯酶。
應用
蛋白酶抑制剂混合液 Set I – Calbiochem 已用于裂解缓冲液中以裂解细胞并进行蛋白免疫印迹。
Protease Inhibitor Cocktail Set I have been used as a supplement in:
- cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
- RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
- sucrose solution to homogenize mice cortices for myelin isolation
生化／生理作用
Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.
特點和優勢
- 蛋白酶抑制剂混合液保护粗提取物中的目标蛋白免于分解
- 可立即用于重构（reconstitution）
警告
毒性：有害（C）
重構
复溶后，等分并冷冻保存（-20°C）。贮备溶液在-20°C下可稳定保存至多1个月。
用1 ml dH2O复溶每个小瓶，以获得100X的贮备溶液。
其他說明
如果直接用IMAC树脂处理细胞提取物，EDTA 的存在可能干扰His•Tag 融合蛋白的纯化。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
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