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重要文件

C2522

Sigma-Aldrich

碳酸酐酶同工酶 II 来源于牛红细胞

lyophilized powder, ≥3,000 W-A units/mg protein

同義詞:

碳酸水解酶, 碳酸盐脱水酶, 碳酸酐酶 II

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

bovine erythrocytes

品質等級

形狀

lyophilized powder

比活性

≥3,000 W-A units/mg protein

分子量

30 kDa

pI 

~5.9

儲存溫度

−20°C

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應用

Sigma 公司的碳酸酐酶已被用于通过测量结合亲和力和化学计量学来分析酶的热力学稳定性。在大鼠自身免疫性胰腺炎研究期间,还使用该酶生成了对碳酸酐酶特异性的 CD 4 + T 细胞系。

生化/生理作用

碳酸酐酶是一种锌金属酶,分子量约为 30,000 Da。这种酶催化二氧化碳水合生成碳酸。参与 pH 和 CO 2 动态平衡、碳酸氢盐和 CO 2 的转运、生物合成反应、骨吸收、钙化、致瘤等许多生理和病理过程。因此,阻断该酶的抑制剂可能对青光眼、癫痫和帕金森氏病具有治疗意义。

單位定義

一个 Wilbur-Anderson (W-A) 单位每分钟将导致 0.02 MTrizma 缓冲液的 pH 值在 0°C 下从 8.3 降至 6.3(一个 W-A 单位基本上相当于一个 Roughton-Booth 单位)。

抑制劑

產品號碼
描述
訂價

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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Nature communications, 9(1), 328-328 (2018-01-25)
In clathrin-mediated endocytosis, adapter proteins assemble together with clathrin through interactions with specific lipids on the plasma membrane. However, the precise mechanism of adapter protein assembly at the cell membrane is still unknown. Here, we show that the membrane-proximal domains
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ThermoFluor (a miniaturized high-throughput protein stability assay) was used to analyze the linkage between protein thermal stability and ligand binding. Equilibrium binding ligands increase protein thermal stability by an amount proportional to the concentration and affinity of the ligand. Binding
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Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

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