C1261

Carboxypeptidase A−Agarose

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

• 同義詞: Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
• 酶委員會編號: 3.4.17.1 (BRENDA, IUBMB)
• MDL號碼: MFCD00130718
• 分類程式碼代碼: 12352204
• NACRES: NA.54

屬性

• 生物源: enzyme from bovine pancreas
• 形狀: ammonium sulfate suspension
• 比活性: ≥6 units/mL packed gel, 25 °C
• 分子量: ~35,250
• 基質: beaded agarose
• 儲存溫度: 2-8°C

描述

一般說明

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

生化／生理作用

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

單位定義

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

外觀

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

安全資訊

• 象形圖: GHS08,GHS07
• 訊號詞: Danger
• 危險聲明: H315,H319,H334,H335
• 防範說明: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• 危險分類: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 標靶器官: Respiratory system
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable