跳轉至內容
Merck
全部照片(2)

重要文件

B5144

Sigma-Aldrich

菠萝蛋白酶 来源于菠萝的茎

≥4 units/mg protein, (chromatography purified)

登入查看組織和合約定價


About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

(chromatography purified)
≥4 units/mg protein

成份

Protein, 30-50% biuret

溶解度

H2O: soluble 1.0 mg/mL, clear, colorless

儲存溫度

−20°C

尋找類似的產品? 前往 產品比較指南

應用

来自菠萝茎的菠萝蛋白酶已被用作十二烷基硫酸钠(SDS)/聚丙烯酰胺凝胶电泳(PAGE)酶谱中的阳性对照。它还被用作测定蛋白水解活性的偶氮酪蛋白底物。
菠萝蛋白酶可用于抑制促炎前列腺素的生物合成。可用于降低凝血效率。来自菠萝茎的菠萝蛋白酶已被用于制备蜜蜂采集的花粉的酶水解产物

生化/生理作用

菠萝蛋白酶是一种半胱氨酸内肽酶,对蛋白质的裂解具有广泛的特异性。菠萝蛋白酶可能来自干或水果。产品 P3169(pGlu-Phe-Leu-P-硝基苯胺)可用作菠萝蛋白酶含量测定的显色底物。

單位定義

25 °C 下 pH 4.6 下,1 个单位每分钟将从 N-α-Z-L-赖氨酸对硝基苯酯中释放 1.0 μmol 对硝基苯酚。

外觀

含甘露醇和磷酸钾缓冲盐的冻干粉

準備報告

色谱纯化。溶于 1 mg/mL 浓度的水中,形成澄清、无色溶液。
色谱纯化产品

基底

產品號碼
描述
訂價

抑制劑

產品號碼
描述
訂價

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

未看到正確版本?

如果您需要一個特定的版本,您可以透過批號來尋找特定憑證。

已經擁有該產品?

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
H Umesh Hebbar et al.
Bioresource technology, 99(11), 4896-4902 (2007-10-30)
Reverse micellar systems of CTAB/isooctane/hexanol/butanol and AOT/isooctane are used for the extraction and primary purification of bromelain from crude aqueous extract of pineapple wastes (core, peel, crown and extended stem). The effect of forward as well as back extraction process
Rubab Mahmood et al.
Biochemistry. Biokhimiia, 72(3), 307-312 (2007-04-24)
Stem bromelain was covalently coupled to a thermosensitive polymer of N-isopropylacrylamide (p(NIPAm)) either through the amino groups of the enzyme (randomly coupled) or via the lone oligosaccharide chain (uniformly coupled). The enzyme coupled via the oligosaccharide chain exhibited better access
Hafeeza Khatoon et al.
Protein and peptide letters, 14(3), 233-236 (2007-03-10)
The lone oligosaccharide chain of stem bromelain was oxidized with periodic acid to generate aldehyde groups and the resulting oxidized enzyme coupled to amino-Sepharose in order to obtain an immobilized preparation with uniformly oriented enzyme. The immobilized bromelain exhibited high
Soghra Khatun Haq et al.
The international journal of biochemistry & cell biology, 37(2), 361-374 (2004-10-12)
The effect of salts and alcohols was examined on the partially folded intermediate (PFI) state of stem bromelain reported at low pH (Haq, Rasheedi, and Khan (2002) European Journal of Biochemistry 269, 47-52) by a combination of optical methods like

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務